A protic ionic liquid attenuates the deleterious actions of urea on α-chymotrypsin.
نویسندگان
چکیده
We present a biocompatible ionic liquid, triethyl ammonium acetate (TEAA), that attenuates the denaturation action of a non-ionic chaotrope, urea, on the industrially relevant proteolytic enzyme α-chymotrypsin.
منابع مشابه
Water and a protic ionic liquid acted as refolding additives for chemically denatured enzymes.
In this communication, we present the ability of water and a protic ionic liquid, triethyl ammonium phosphate (TEAP) to act as refolding additives for the urea-induced chemical denaturated state of the two enzymes, α-chymotrypsin and succinylated Con A. We show that the enzymatic activity is regained and in certain circumstances enhanced.
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ورودعنوان ژورنال:
- Physical chemistry chemical physics : PCCP
دوره 13 38 شماره
صفحات -
تاریخ انتشار 2011